Two Mutations Are Better than One
色情视频 biologists find that fruit flies with two muscle protein mutations do better than those with a single mutation, suggesting a new view of human heart disease.
Two wrongs don鈥檛 make a right, but in the case of genetic mutations, having two mutations in the same gene could be better than having either one individually. Recent research by biologists at 色情视频 found that two separate genetic modifications each greatly reduced the function of the myosin muscle protein in fruit flies, but flies with both mutations had nearly three-quarters of the protein function restored. The findings are important for researchers looking to better understand and treat heart muscle disease in humans.
Myosin is a motor protein involved in muscle contraction. The proper functioning of this protein depends on a series of chemical bonds that hold the protein in its proper configuration. Mutating the protein to destroy some of these bonds can cause the protein to lose some or all of its function, leading to abnormal muscle contraction in the organism.
Earlier studies had suggested that within the myosin protein, the interaction between two amino acid subunits called E497 and R712 is charge-dependent. In other words, in order to normally function, a negative charge on one amino acid interacts with a positive charge on the other. In humans, mutations in either of these amino acids can result in hypertrophic cardiomyopathy, a common form of inherited heart muscle disease. In a recent study, investigators at 色情视频 looked further into how exactly disrupting the charges of these amino acids might lead to muscle defects.
Mutant muscles
色情视频 biologist and the study's corrsponding author Sanford Bernstein, along with coauthors William Kronert, Girish Melkani and Anju Melkani, experimented with fruit flies with genetically modified proteins in the flies鈥� flight muscles to see what effects that would have on the proteins鈥� functioning. The researchers bred two versions of fruit flies in which either the fly version of the E497 or the R712 myosin protein amino acids were mutated by having their charges reversed. Those flies lost their flight ability completely. Using a combination of biochemical techniques, the researchers found that the myosin proteins were about five times less active in these flies.
A third line of genetically modified flies had the charges reversed in both E497 and R712 myosin protein amino acids. These flies still couldn鈥檛 fly, but the researchers found that the proteins were about 73-percent as active as those in normal, unmodified flies鈥攁 vast improvement over having either single mutation by itself.
Interestingly, heterozygous flies (those with copies of both normally functioning and genetically modified genes) in the study did manage to fly when they were young. However, this occurred only for the double-mutant. Overall, the double-mutation restored most of the protein activity that was lost in the other two mutant lines, demonstrating that both amino acids need to interact in a charge-dependent manner in order for normal muscle protein function to occur. The researchers earlier this month in the Journal of Biological Chemistry.
Helping humans
Because the myosin protein modified in the flies is so similar to the one known to be involved in some human cardiomyopathy, and since the same amino acids are affected, Bernstein said that a similarly dysfunctional process probably underlies the disease caused by mutations in these specific amino acids in humans.
鈥淚n humans, it鈥檚 a different type of mutation, but it鈥檚 one that we predict is disruptive to normal interaction within the protein, resulting in decreased or possibly increased myosin function,鈥� he said.
For that reason, researchers can further study these proteins in flies to learn more about the disease. Bernstein said that the team鈥檚 results suggest that the abnormal function in human heart muscle cells is largely a result of structural failings in the myosin protein that lead to biochemical dysfunction and an unsuccessful attempt to compensate for these defects, resulting in abnormal heart structure and function.
What鈥檚 more, the findings point to the potential for drugs that can modify myosin function to overcome connection errors within abnormally charged myosin proteins, which might improve functioning in human heart muscle cells.
色情视频 is home to several research programs looking into ways to improve and restore heart function, including the 色情视频 Heart Institute, which explores the potential of cardiac stem cells, and the Donald P. Shiley BioScience Center, which is investigating a number of topics surrounding cardiovascular disease.
