色情视频

鈥淕etting a genome tells you how the animal works.鈥�

Using a tiny tissue sample collected from one of the largest fishing ports in India, a 色情视频 laboratory has developed a detailed genetic profile of a vulnerable shark species鈥攁 step that could prove useful in future conservation efforts.

Conservation geneticist Shaili Johri sequenced the genome of the silky shark, known for the distinctive texture of its skin and a particularly acute sense of hearing. It鈥檚 only the sixth new genome out of 1,200 species of sharks, providing data needed for biological conservation and management that was previously unavailable.

鈥淕etting a genome tells you how the animal works,鈥� said marine microbial ecologist Elizabeth Dinsdale, head of a lab in the Engineering and Interdisciplinary Sciences building where the work was completed.

Johri used a DNA sequencing instrument the size of a flip phone. Invented primarily as a medical diagnostic tool, the $1,000 device connects with a laptop computer to perform a genome 鈥渟kimming,鈥� a less complete version of the kind of work that would typically cost nearly $3 billion during the days of the Human Genome Project.

Today, genome sequencing is a speedier process. Because of this, researchers were able sequence the DNA of the shark in about three days and to annotate鈥攖he process of identifying the locations of the genes and what they do鈥攊n  about a year.

Johri, an adjunct faculty member and post-doctoral student in Dinsdale鈥檚 lab with a personal interest in wildlife conservation, traveled to 8,700 miles to Veraval, India, to collect DNA samples and assess shark biodiversity. The sharks are mostly bycatch, collected while fishing for tuna and mackerel.

Tissue samples

Johri worked with collaborators from the regional College of Fisheries to act as liaisons with local fishers and sellers, obtain samples of muscle tissue as small as 3 millimeters from shark fins coming into the port and sold at fish and export markets.

The shark was sequenced on a hand-held device, the MinION, manufactured by U.K.-based Oxford Nanopore Technologies. The technology allows the sequencing to be performed  in the field through a connection to a portable computer; it has been used in remote field labs to sequence DNA from an Ebola virus outbreak.

The source of the fins wasn鈥檛 known at first; identifying a specific shark typically requires an entire body, Johri said. The sequencing, however, allowed her to identify one of the specimens as the silky shark (Carcharhinus falciformis) which swims in tropical waters and lives off tuna, octopus, and squid.

鈥淲hat we show in this method is that it was able to sequence very different kinds of DNA within the silky shark genome,鈥� Johri said, 鈥渟o that increases the likelihood that it would be able to sequence other species equally well.鈥�

The silky shark is not endangered, but is considered vulnerable due to the continued market for its oil and fins and a slow reproductive rate. And many similar species do face extinction. The ability to identify a shark species from genes in a small sample could help detect illegal trade and promote conservation management.

鈥淪harks are an important organism,鈥� Dinsdale said. 鈥淩emoving sharks has significant effects on all the other components of the environment.鈥�

New data

Identifying the genome provides information to learn more about the sharks鈥� numbers, where they live and their evolutionary development, filling an ecological 鈥渄ata deficiency鈥� that applies to about half of all shark species. The new data then could be used to designate protected areas and take other steps to manage fishing.

While this research used actual tissue samples, Johri said the lab is testing the feasibility of gathering DNA just from ocean water, capturing it from shed cells or fecal matter of organisms that have passed by.

The product of the research is not yet a complete genome, Dinsdale said, 鈥渂ut it鈥檚 a quite good partial genome鈥� and it corrects a previous effort that was found to be riddled with mistakes.

Beyond the use of the genetic knowledge in shark conservation, Dinsdale noted there are bigger science questions the study could help answer. Sharks show an apparently high genome stability that is a factor in their long life spans, great wound-healing powers and a near-immunity to cancer.

on the work was published in Nature Scientific Reports. Funding for the research came from a private donor and the international Society for Conservation Biology-Marine Section.

Sequencing the silky shark The Dinsdale lab at 色情视频 sequenced the genome of the silky shark for the first time.